Developmental Biology
of the
Market Squid
![](https://static.wixstatic.com/media/d16ed3_20ea6c4dd167f9fd91159d296b71fb4d.png/v1/fill/w_272,h_205,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/d16ed3_20ea6c4dd167f9fd91159d296b71fb4d.png)
![](https://static.wixstatic.com/media/d16ed3_da2848199aeaa83759ff290f98929f66.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_da2848199aeaa83759ff290f98929f66.jpg)
Early stage squid embryos
![](https://static.wixstatic.com/media/d16ed3_9f7f46d02d53c59720e42b7a99a63930.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_9f7f46d02d53c59720e42b7a99a63930.jpg)
Developing eye
![](https://static.wixstatic.com/media/d16ed3_fa47869a98bc098d0ca5d48773288782.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_fa47869a98bc098d0ca5d48773288782.jpg)
Early mantle and anterior siphon folds
![](https://static.wixstatic.com/media/d16ed3_6c55c948c37ee60427b4842edafc3343.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_6c55c948c37ee60427b4842edafc3343.jpg)
Early stage squid embryos
![](https://static.wixstatic.com/media/d16ed3_5cadb10437e677b24d8233493c153cc1.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_5cadb10437e677b24d8233493c153cc1.jpg)
Developing eye
![](https://static.wixstatic.com/media/d16ed3_b9165dda3984d46100ada7972849caea.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_b9165dda3984d46100ada7972849caea.jpg)
Early mantle and anterior siphon folds
![](https://static.wixstatic.com/media/d16ed3_81c03e3c062b7ed1251364d8968e17c6.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_81c03e3c062b7ed1251364d8968e17c6.jpg)
Chromatophores (dark spots) on the embryo's mantle
![](https://static.wixstatic.com/media/d16ed3_ace68ede9e87ffa49a2f66d13133d5dd.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_ace68ede9e87ffa49a2f66d13133d5dd.jpg)
Eye and tentacles. Notice the musculature around the chromatophores
![](https://static.wixstatic.com/media/d16ed3_8f097adfb607e7a41d24dcd549f03c9e.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_8f097adfb607e7a41d24dcd549f03c9e.jpg)
The blastoderm surrounds the external yolk sac.
![](https://static.wixstatic.com/media/d16ed3_9ccc5dcff9dbc900c3b8643080c395a6.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_9ccc5dcff9dbc900c3b8643080c395a6.jpg)
Later stage squid embryo
![](https://static.wixstatic.com/media/d16ed3_9b45a51a617484c4c200547f3b82d954.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_9b45a51a617484c4c200547f3b82d954.jpg)
Eye and tentacles
![](https://static.wixstatic.com/media/d16ed3_b106af1869201f82d2238b1243f4e546.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_b106af1869201f82d2238b1243f4e546.jpg)
Cell nuclei covering yolk sac
![](https://static.wixstatic.com/media/d16ed3_bfdbd84b35775e0c72f67294562b54ed.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_bfdbd84b35775e0c72f67294562b54ed.jpg)
Later stage squid embryo
![](https://static.wixstatic.com/media/d16ed3_8543403285c21bb8b482bb1a366a0b22.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_8543403285c21bb8b482bb1a366a0b22.jpg)
![](https://static.wixstatic.com/media/d16ed3_ca4fb4a411bf8ddc600686a906229af8.jpg/v1/fill/w_147,h_110,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/d16ed3_ca4fb4a411bf8ddc600686a906229af8.jpg)
Immunofluorescence
Two stages of developing embryos were stained with florescent molecules and antibodies to observe different tissues. DAPI (4',6-diamidino-2-phenylindole) stains cell nuclei a fluorescent blue. Phalloidin binds to actin and was therefore used to stain muscles cells. Phalloidin stains bright green and the brightest areas are the ones with the most muscles tissue. Antibodies raised against serotonin were used to stain nerve cells, but unfortunately the cells were not visible, possibly because of the thickness of the embryos.